In: Phyton 57 (2017): S. 69-78 DOI: 10.12905/0380.phyton57-2018-0069 Published online on 12th February 2018
Published online on 12th February 2018
Shoot regeneration and isoenzyme expression of Moringa oleifera under the influence of salt stress
Jehan M. Salem, Ahmed M. Hassanein, Fayza A. Faheed & Abdullah A. El-nagish
Key words: Shoot regeneration, shoot multiplication, isoenzyme expression, cytokinins, gene expression, salt stress, salinity, Moringa oleifera.
Salem J. M., Hassanein A. M., Faheed F. A. & El-nagish A. A. 2018. Shoot regeneration and isoenzyme expression of Moringa oleifera under the influence of salt stress. – Phyton (Horn, Austria) 57 (1–2): 69–78, with 7 figures.
Shoot regeneration and isoenzyme expression of Moringa oleifera under the influence of cytokinin type and different concentrations of NaCl were studied. Cotyledonary nodal segments obtained from in vitro grown seedlings (initial culture) and nodal segments obtained from their regenerants (first subculture) were subjected to shoot formation on MS medium supplemented with 0.56 mg/l BAP or KIN, for three weeks each. Generally, the number of regenerated shoots was higher under the influence of BAP than under the influence of KIN, on cotyledonary nodal segments as well as during the two successive subcultures. On MS with BAP, a high number of vigor shoots was associated with the formation of callus on the base of explants. During the initial culture, NaCl exerted a concentration-dependent negative effect on shoot formation. The presence of NaCl during the initial culture improved regeneration during the first subculture (up to 4 g/l NaCl) and increased the expression of peroxidases, superoxide dismutases and esterases. On the other side, the expression of glutamate oxaloacetate transaminases was decreased. This indicated that M. oleifera shoots increased the expression of these enzymes to control the negative effect of NaCl on shoot regeneration and on the growth of regenerated shoots.