In: Phyton 58, Fasc. 2 (2018): S. 165-174 DOI: 10.12905/0380.phyton58(2)-2018-0165 Published online on 21st December 2018
Published online on 21st December 2018
A micropropagation protocol for the domestication of
Jacaranda ulei (Bignoniaceae)
Conceição Eneida dos Santos Silveira, Wagner S. Fukuda, Dario Palhares,
Natália Faustino Cury & Luís Alfredo Rodrigues Pereira
Key words: Micropropagation, seed disinfestation, in vitro cultivation, domestication, Cerrado, carobinha, Jacaranda ulei.
Silveira C. E. S., Fukuda W. S., Palhares D., Cury N. F. & Pereira L. A. R. 2018. A micropropagation protocol for the domestication of Jacaranda ulei (Bignoniaceae). – Phyton (Horn, Austria) 58 (2): 165–174, with 4 figures and 4 tables.*
Jacaranda ulei is a Cerrado subshrub with ethnobotanical and ornamental relevance. J. ulei seeds were disinfested, germinated and cultivated and multiplied in ¼ MS media containing sucrose (20 g·l-1), agar (8 g·l–1), and combinations of BAP (6-Benzylaminopurine) and IBA (Indole-3-butyric acid) growth regulators. Subsequently, the explants were rooted under in vitro and ex vitro conditions, and then acclimatized. J. ulei seeds had a high germination rate (>90 %) and did not manifest a significant percentage of abnormal seedlings. Furthermore, there was no effect of growth regulators or
subcultures on the number of shoot sprouts, which ranged from 1.0 to 2.0 sprouts per explant. Similarly, there was no consistent effect of growth regulators on the explant length. Nonetheless, a subculture effect on shoot length was observed, which reached a mean of 2.3 cm in the third subculture. For rooting, the ex vitro conditions were the best, showing longer and branched adventitious roots than under in vitro conditions. Transverse sections of the root insertion region showed that the vasculature was directl connected to the stem vascular system. Besides, the acclimatized 24-month-old plants formed a primary xylopodium, which was able to sprout and produce new shoots.